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US Series-A VCs investing in B2B startups

A curated list of active US Series-A VCs investing in B2B startups.

by Tzakhi Freedman · March 16, 2026


Today, we gathered 10 selected listings from our investor database. Our team reviewed, checked, and enriched the list with the necessary data.

Here goes:

  • Arthur Ventures, Minneapolis venture capital firm investing at the A-round stage in B2B software companies. Arthur Ventures focuses on early-stage opportunities and backs founders building technology businesses located outside Silicon Valley. The firm concentrates on supporting B2B software startups as they scale products and operations during the early growth phase.
  • Avenue Growth Partners, Washington venture capital firm investing at the A-round stage in B2B software companies. Avenue Growth Partners provides growth capital and targets high-growth businesses at the expansion stage. The firm focuses on scaling B2B technology companies, partnering with founders as they accelerate revenue and expand their market presence.
  • Jurassic Capital, Durham venture capital firm investing at the A-round stage in B2B software companies. Jurassic Capital focuses on growth-stage opportunities and backs technology businesses building enterprise software products. The firm partners with founders of B2B companies seeking capital and support to expand operations and accelerate growth.
  • Lewis & Clark Ventures, St Louis venture capital firm investing at the A-round stage in B2B software companies. Lewis & Clark Ventures operates as an early-stage tech fund supporting founders building enterprise technology products. The firm concentrates on B2B software opportunities and partners with startups as they develop and scale innovative solutions.
  • Lorimer Ventures, Brooklyn venture capital firm investing at the A-round stage in B2B companies. Lorimer Ventures backs entrepreneurs building B2B businesses and focuses on supporting founders pursuing ambitious technology opportunities. The firm partners with teams developing enterprise products and works with them as they grow and expand their companies.
  • Method Capital, Chicago venture capital firm investing at the A-round stage in B2B technology companies. Method Capital specializes in growth-stage investments and focuses on supporting businesses building enterprise technology products. The firm targets B2B companies as they scale operations and develop solutions for business customers.
  • PHX Ventures, Phoenix venture capital firm investing at the A-round stage in B2B SaaS companies. PHX Ventures focuses exclusively on software businesses and is described as Arizona’s most active software investor. The firm backs founders building SaaS products for business customers and partners with companies during their early growth stage.
  • ValueStream Ventures, New York venture capital firm investing at the A-round stage in B2B, fintech, and software companies. ValueStream Ventures operates as a thesis-driven early-stage firm investing in software, data and analytics, and fintech businesses. The firm focuses on supporting founders building enterprise technology platforms and scalable B2B products.
  • Vertex Ventures US, Palo Alto venture capital firm investing at the A-round stage in B2B SaaS companies. Vertex Ventures US partners with early-stage founders building infrastructure and SaaS technology businesses. The firm focuses on supporting enterprise software startups as they develop products and scale platforms serving business customers.
  • Zelkova Ventures, Miami venture capital firm investing at the A-round stage in B2B SaaS companies. Zelkova Ventures focuses on early-stage venture investments and partners with entrepreneurs building enterprise software products. The firm supports founders developing B2B SaaS companies and works with them to build and grow technology businesses.

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